Evaluation of the antibacterial effects of Arctium lappa extracts on Brucella melitensis 16M in the animal model and macrophage culture

Document Type : Original Research

Authors

1 Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran

2 Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran

3 Young Researchers and Elite Club, Zanjan Branch, Islamic Azad University, Zanjan, Iran

Abstract

Aims: The aim of this study was to evaluate the antibacterial effects of aqueous and organic extracts of Arctium lappa on Brucella melitensis 16M in vitro, in macrophage culture and in the animal model.
Methods: In this experimental study aquatic, ethanolic, and acetonic extracts of Arctium lappa were prepared. The antibacterial effect of the extracts investigated by agar well diffusion and macrodilutionon methods for determination of MIC and MBC. Then the intramacrophge survival of the B.melitensis 16M was studied from the cell culture of Balb/c mice. Also, the antibacterial effect of the extracts were studied in the animal model.
Results: The MIC and MBC of leaves and inflorescences extracts of Arctium lappa on B. melitensis 16 M were 101 and 116 mg/ml, respectively. The maximum inhibition zone diameter was related to an ethanolic extract. In the macrophage culture and in the animal model, the aquatic extract of inflorescences of Arctium lappa was the most effective.
Conclusion: The aquatic and organic extracts of Arctium lappa have antibacterial activity against intramacrophage B. Melitensis 16M. The aquatic extract has the most effective antimicrobial activity on intramacrophage B. Melitensis 16M. These extracts can be useful in the treatment of brucellosis.

Keywords

Journal of Military Medicine
Volume 18, Issue 1 - Serial Number 63
May and June 2016
Pages 315-322

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