Histopathologic changes in lung rat of the acute and chronic exposure sulfor mustard

Document Type : Original Research

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Abstract

the aim of this study evaruated the effects of surfure mustard (SM) on alveolar, lung paranchym and designed lung chemical agent exposure animal model. Seventhy eight NMRI 3 months old
Rats (weight 200±40g) were serected and randomJy divided groups sham, control and experimental based on duration of exposure and dosage of intraperitoneal injection (IP). Sham and groups recieved thyrods buffer (surfur rnustard solution) or nothing respectively. Five groups, received once different doses of SM (2.5/5/l0/20/40 mg/kg). All experimental groups, for evaluation of subacute and chronic stage, different doses of SM injected and after 24 hours, 2
weeks and 8 weeks misecared. Dissected and 5 samples were taken from right lung tissue. All samples were fixed, embeded in parafin wax and serially sectioned in 5 micron pieces by microtome Then they were stained by Hematoxylin and Eosin. This results showed that the effects
of SM an arveolar and paranchym depend on doses and remain for along time. Among experiments groups, Lesion of alveolar structure and paranchym were the highest than sham and control groups. Too Depending on doses, Histopathological changes such as edema, inflamation and hemorage
(less in 2.5mg/kg and highest in 40mg/kg) were observed. In conclusion this results showed that, the SM expousre lung rat good animal model for study of
effects of SM on lung tissue.

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Journal of Military Medicine
Volume 5, Issue 3 - Serial Number 13
September and October 2003
Pages 211-217

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